Publications
Publications
Embedded Files
Journal Articles
1. Frías Beneyto R, Tiebosch I., Costa A., Kylmann Hansen D., Holmberg Å., Vlasbom R., Withfield L., Degryse A.-D., Prins J.-B. (submitted). A Framework for Assessing Practical Competence in Laboratory Animal Science (LAS): Development of Direct Observation of Practical Skills (DOPS) by the ETPLAS Working Group.
2. Pernold K, M Andersson, Lilja N, Frias Beneyto R, Ulfhake B. Longitudinal Study of Changes in Ammonia, Carbon Dioxide, Humidity and Temperature in Individually Ventilated Cages Housing Female and Male C57BL/6N Mice during Consecutive Cycles of Weekly and Bi-Weekly Cage Changes. Animals 2024, 14(18), 2735; https://doi.org/10.3390/ani14182735
3. CJ Martel-Benítez, R Alayón-Afonso, D Padilla Castillo, F J Chamizo-López, M Isabel García-Laorden, A Espinosa de Los Monteros Y Zayas, JC Rivero-Vera, P Nogueira Salgueiro, F Real, A Bordes-Benítez, A Martel Quintana, C Almeida Peña, C Domínguez Cabrera, JM González-Martín, J Martín Caballero, R Frías Beneyto, Jesús Villar, JL Martín-Barrasa. Long-Term Treatment with Alcaligenes faecalis A12C Improves Host Resistance to Pathogens in Septic Rats: Possible Contribution of Curdlan-Like Immune Trainer. Probiotics Antimicrob Proteins. 2024 Apr 26. doi: 10.1007/s12602-024-10252-0
4. Van Beuningen N, Alkema S, Hijlkema N, Ulfhake B, Frias R, Ritskes-Hoitinga M, Alkema W. The 3Ranker: An AI-based Algorithm for Finding Non-animal Alternative Methods. Altern Lab Anim. 2023 Oct 21:2611929231210777. doi: 10.1177/02611929231210777
5. Frias R. Let’s do it right: 8 steps to competence in laboratory animal science in the European Union. Laboratory Animals Journal. 2023 Mar 24;236772231157455. doi: 10.1177/00236772231157455. PMID: 36960677
6. Gutiérrez‑Falcón AI, M. Ramos‑Nuez A, Espinosa de los Monteros y Zayas A, Padilla Castillo DF, García‑Laorden MI, Chamizo‑López FJ, Real Valcárcel F, Artilles Campelo F, Bordes Benítez A, Nogueira Salgueiro P, Domínguez Cabrera C, Rivero‑Vera JC, González‑Martín JM, Martín Caballero J, Frías‑Beneyto R, Villar J, Martín‑Barrasa JL. Probiotic Properties of Alcaligenes faecalis Isolated from Argyrosomus regius in Experimental Peritonitis (Rat Model). Probiotics & Antimicro. Prot. (2021). https://doi.org/10.1007/s12602-021-09767-7
7. Ortín-Piqueras V, Freitag TL, Andersson LC, Lehtonen SH, Meri SK, Spillmann T, Frias R. Urinary Excretion of Iohexol as a Permeability Marker in a Mouse Model of Intestinal Inflammation: Time Course, Performance and Welfare Considerations. Animals. 2021 Jan 4;11(1):79. doi: 10.3390/ani11010079. PMID: 33406796 PMCID: PMC7824797 DOI: 10.3390/ani11010079.
8. Wilhelm FR, Krautwald-Junghanns ME, Ortín Piqueras V, Cramer K, Forsgård RA, Frias R, Spillmann T, Schmidt V. Iohexol-based measurement of intestinal permeability in birds. Journal of Exotic Pet Medicine. Vol 34, July 2020, pages 18-23. DOI: https://doi.org/10.1053/j.jepm.2020.04.004
9. Forsgård RA, Marrachelli VG, Lindén J, Frias R, Collado MC, Korpela R, Monleon D, Spillmann T, Österlund P. Two-Week Aflibercept or Erlotinib Administration Does Not Induce Changes in Intestinal Morphology in Male Sprague–Dawley Rats But Aflibercept Affects Serum and Urine Metabolic Profiles. Translational Oncology Vol 12, Issue 8, 1122-1130, 2019. PMID: 31176994 PMCID: PMC6558216 DOI: 10.1016/j.tranon.2019.04.019
10. Watson VE, Jacob ME, Bruno-Bárcena JM, Amirsultan S, Stauffer SH, Ortín Píqueras V, Frias R, Gookin JL. Influence of the intestinal microbiota on disease susceptibility in kittens experimentally infected with atypical enteropathogenic Escherichia coli. Veterinary Microbiology 2019, Vol 231: 197-206. PMID: 30955810 PMCID: PMC6532395 DOI: 10.1016/j.vetmic.2019.03.020
11. Ortín Piqueras V, Spillmann T, Sankari S, Pöytäkangas M, Frias R. Determination of iohexol in canine plasma – strong correlation between enzyme-linked immunosorbent assay, high performance liquid chromatography, and neutron activation analysis. Scand. J. Lab. Anim. Sci. 2018, 44, 4, 1-7. DOI: https://doi.org/10.23675/sjlas.v44i0.535
12. Forsgård RA, Marrachelli VG, Korpela K, Frias R, Collado MC, Korpela R, Monleon D, Spillmann T, Österlund P (2017). Chemotherapy-induced gastrointestinal toxicity is associated with changes in serum and urine metabolome and fecal microbiota in male Sprague-Dawley rats. Cancer Chemother Pharmacol 2017, 80, 2, 317-332. PMID: 28646338 PMCID: PMC5532424 DOI: 10.1007/s00280-017-3364-z
13. Gómez-Gallego C, García Romo M, Frias R, Periago MJ, Ros G, Salminen S, Collado MC (2017). Mice exposed to infant formula enriched with polyamines: impact on host transcriptome and microbiome. Food & Function. Royal Society of Chemistry. PMID: 28294281 DOI: 10.1039/c7fo00073a
14. Forsgård RA, Korpela R, Holma R, Lindén J, Frias R, Spillmann T, Österlund P (2016). Intestinal permeability to iohexol as an in vivo marker of chemotherapy-induced gastrointestinal toxicity in Sprague-Dawley rats. Cancer Chemotherapy and Pharmacology. 2016 Oct;78(4):863-74. PMID: 27590707 PMCID: PMC5040744 DOI: 10.1007/s00280-016-3150-3
15. Kalliokoski S, Ortín Piqueras V, Frias R, Sulic A M, Määttä J AE, Kähkönen N, Viiri K, Huhtala H, Pasternack A, Laurila K, Sblattero D, Korponay-Szabó IR, Mäki M, Caja S, Kaukinen K, Lindfors K (2016). Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice. Amino Acids, Aug 9, 2016. PMID: 27503559 DOI: 10.1007/s00726-016-2306-0
16. Kalliokoski S, Caja S, Frias R, Laurila K, Koskinen O, Niemelä O, Mäki M, Kaukinen K, Korponay-Szabó IR, Lindfors K (2015). Injection of celiac disease patient sera or immunoglobulins to mice reproduces a condition mimicking early developing celiac disease. Journal of Molecular Medicine 2015 Jan;93(1):51-62. PMID: 25209899 DOI: 10.1007/s00109-014-1204-8
17. Salles Teixeira TF, Boroni Moreira AP, Silva Souza CN, Frias R and Gouveia Peluzio MC (2014). Intestinal permeability measurements: general aspects and possible pitfalls. Nutricion Hospitalaria, Vol 2 Issue 29, 269-281. PMID: 24528342 DOI: 10.3305/nh.2014.29.2.7076
18. Gómez-Gallego C, Frias R, Pérez-Martínez G, Bernal MJ, Periago MJ, Salminen S, Ros G, Collado MC (2014). Polyamine supplementation in infant formula: influence on lymphocyte populations and immune system-related gene expression in a Balb/cOlaHsd mouse model. Food Research International 59 (2014) 8-15. DOI: 10.1016/j.foodres.2014.01.066
19. Gómez-Gallego C, Collado MC, Pérez G, Ilo T, Jaakkola U-M, Bernal MJ, Periago MJ, Frias R, Ros G, Salminen S (2014). Resembling breast milk: influence of polyamine-supplemented formula on neonatal BALB/cOlaHsd mouse microbiota. British Journal of Nutrition (2014), 111: 1050-1058. PMID: 24229796 DOI: 10.1017/S0007114513003565
20. Frias R, Ouwehand AC, Jaakkola U-M, Spillmann T and Gueimonde M (2014). An in vivo permeability test protocol using iohexol to reduce and refine the use of laboratory rats in intestinal damage assessment. Scand. J. Lab. Anim. Sci. 2014, Vol 40, Number 1. DOI: https://doi.org/10.23675/sjlas.v40i0.320
21. Gomez-Gallego C, Ilo T, Jaakkola U-M, Salminen S, Ros G and Frias R (2013). A method to collect high volumes of milk from mice. An. Vet. (2013) 29: 55-61. I.S.S.N.: 0213-5434.
22. Kalliokoski S, Sulic AM, Korponay-Szabó IR, Szondy Z, Frias R, Perez MA, Martucciello S, Roivainen A, Pelliniemi LJ, Esposito C, Griffin M, Sblattero D, Mäki M, Kaukinen K, Lindfors K, Caja S (2013). Celiac Disease-Specific TG2-Targeted Autoantibodies Inhibit Angiogenesis Ex Vivo and In Vivo in Mice by Interfering with Endothelial Cell Dynamics. PLoS One. Jun 18;8(6): e65887. PMID: 23824706 PMCID: PMC3688809 DOI: 10.1371/journal.pone.0065887
23. Gómez-Gallego C, Collado MC, Ilo T, Jaakkola, U-M, Bernal MJ, Periago MJ, Salminen S, Ros G, Frias R (2012). Infant formula supplemented with polyamines alters the intestinal microbiota in neonatal BALB/cOlaHsd mice. Journal Nutritional Biochemistry Nov; 23(11):1508-13. PMID: 22402370 DOI: 10.1016/j.jnutbio.2011.10.003
24. Frias R, Steiner JM, Williams DA, Sankari S, and Westermarck E. (2012). Urinary recovery of orally administered 51chromium-labeled ethylenediamine tetra-acetic acid, lactulose, rhamnose, D-xylose, 3-O-methyl-D-glucose and sucrose in healthy adult male laboratory Beagles. Am J Vet Research, May, Vol 73, No. 5. PMID: 22533396 DOI: 10.2460/ajvr.73.5.654
25. Frias R, Strube K, Ternes W, Collado MC, Spillmann T, Sankari S, Westermarck E (2012). Comparison of 51Cr-EDTA and iohexol as blood markers for intestinal permeability testing in Beagle dogs. The Veterinary Journal April; 192 (1): 123-5. PMID: 21652237 DOI: 10.1016/j.tvjl.2011.04.024
26. Frias R, Collado MC, Spillmann T, Sankari S, Westermarck E (2010). The 51-chromium-labeled ethylenediamine tetra-acetic acid intestinal permeability blood test in healthy Beagle dogs: a comparison between serum and plasma levels. J Vet Diagn Invest November; Vol. 22, Number 6. PMID: 21088181 DOI: 10.1177/104063871002200616
27. Frias R, Harmoinen J, Laitinen-Vapaavuori O, Spillmann T, Sankari S, Westermarck E (2010). Small intestinal permeability and serum folate and cobalamin absorption after surgical construction of permanent jejunal fistulas in laboratory Beagle dogs. Comparative Medicine. Vol 60, No 5, October, pages 1-5. PMID: 21262122 PMCID: PMC2958205
28. Pöytäkangas M, Saario-Paunio E, Putkonen T, Saastamoinen I, Frias R, Spillmann T and Saloniemi H (2010). Rapid LC-UV Analysis of Iohexol in Canine Plasma for GFR Determination. Chromatographia Feb; 71(3-4): 211-16. DOI: 10.1365/s10337-009-1445-9
29. Klenner S, Frias R, Coenen M, Failing K, Hewicker-Trautwein M, Ternes W, Verspohl J, Spillmann T (2009). Estimation of intestinal permeability in healthy dogs using the contrast medium iohexol. Vet Clin Pathol 38:353-360. PMID: 19351334 DOI: 10.1111/j.1939-165X.2009.00136.x
30. Frias R, Ouwehand AC, Spillmann T, Vankerckhoven V, Hewicker-Trautwein M, Salminen S and Gueimonde M (2009). Effect of clinical and probiotic Lactobacillus rhamnosus strains on intestinal permeability and bacterial translocation in healthy and colitic rats. Food Research International 42 636-640. DOI: 10.1016/j.foodres.2009.02.004
31. Gueimonde M, Frias R and Ouwehand AC (2006). Assuring the continued safety of lactic acid bacteria used as probiotics. Biologia (2006) 61 (6): 755-76061.DOI: 10.2478/s11756-006-0153-2
32. Westermarck E, Frias R, and Skrzypczak T (2005). Effect of diet and tylosin on chronic diarrhea in Beagles. Journal of Veterinary Internal Medicine (2005) 19: 822–827. PMID: 16355675 DOI: 10.1892/0891-6640(2005)19[822:eodato]2.0.co;2
33. Frias R, Sankari S, Westermarck E (2004). 51Cr-EDTA absorption test in serum: an easy method for assessing small intestinal permeability in dogs. Journal of Veterinary Internal Medicine Mar-Apr; 18(2):156-9. PMID: 15058765 DOI: 10.1892/0891-6640(2004)18<156:cabtae>2.0.co;2
34. Harmoinen J, Mentula S, Heikkilä M, van der Rest M, Rajala-Schultz PJ, Donskey CJ, Frias R, Koski P, Wickstrand N, Jousimies-Somer H, Westermarck E, Lindevall K (2004). Orally administered targeted recombinant Beta-lactamase prevents ampicillin-induced selective pressure on the gut microbiota: a novel approach to reducing antimicrobial resistance. Antimicrob Agents Chemother Jan; 48(1): 75-9. PMID: 14693521 PMCID: PMC310163 DOI: 10.1128/aac.48.1.75-79.2004
Abstracts and Conference Proceedings
1. R. Frias. Achieving Competence in Laboratory Animal Science: A Pathway to Animal Welfare and Scientific Excellence. FELASA 16th Congress. Athens, Greece. 2025.
2. R. Frias. Education, Training, and Competence in Laboratory Animal Science. FELASA 16th Congress. Athens, Greece. 2025.
3. R. Frias, L. Whitfield, Ǻ. Holmberg, A. Costa, D.K. Hansen, R. Vlasblom, I. Tiebosch, A.-D. Degryse, J.-B. Prins. DOPS Templates: Supporting Harmonized Assessment of Practical Skills in Animal-Based Procedures. FELASA 16th Congress. Athens, Greece. 2025.
4. R. Frias, Ǻ. Holmberg, A. Costa, D.K. Hansen, I. Tiebosch, R. Vlasblom, L. Whitfield, A.-D. Degryse, J.-B. Prins. ETPLAS Working Group on DOPS Development for Practical Skills Assessment – Introduction and Overview. FELASA 16th Congress. Athens, Greece. 2025.
5. A. Costa, R. Frias, Ǻ. Holmberg, D.K. Hansen, I. Tiebosch, L. Whitfield, A.-D. Degryse, J.-B. Prins. Case studies and real-world application. FELASA 16th Congress. Athens, Greece. 2025.
6. I. Tiebosch, R. Frias, N. Franco. Learning outcomes for a competence assessor. FELASA 16th Congress. Athens, Greece. 2025.
7. I. Tiebosch, R. Frias, A. Costa, Ǻ. Holmberg, D. Kylman Hansen, R. Vlasblom, L. Whitfield, A.-D. Degryse, J.-B. Prins. Understanding the ETPLAS DOPS. FELASA 16th Congress. Athens, Greece. 2025.
8. L. Whitfield, I. Tiebosch, R. Frias, A. Holmberg, A.-D. Degryse, A. Costa, J.-B. Prins. Practical application of the ETPLAS DOPS. FELASA 16th Congress. Athens, Greece. 2025.
9. V. Siderova, K. Edvardsson, A. Lada, R. Frias. Long-term Inhalation Anesthesia with Isoflurane in Sprague Dawley Rats for a Microsurgery Course. FELASA 16th Congress. Athens, Greece. 2025.
10. C. Broddling, A. Lada, R. Frias Beneyto. Transitioning from traditional water bottles to water pouch systems for rodents. FELASA 16th Congress. Athens, Greece. 2025.
11. A.-M. Mikkola-Bergqvist, S. Argyriou Tsikrikoni, C. Broddling, R. Frias Beneyto. Reducing weaning stress in mouse offspring: the benefits of maintaining familiar environments. FELASA 16th Congress. Athens, Greece. 2025.
12. A. Hanberg, K. Dreij, A. Beronius, H. L. Karlsson, E. Wincent, R. Frias, M. Kippler, A. Gliga, M. Olsson, C. Nilsson, J. Zilliacus. Training next-generation toxicologists on the 3Rs. EUROTOX Congress, Copenhagen, Denmark. 2024.
13. The Karolinska Experimental Research and Imaging Center (KERIC) core facility supports longitudinal, terminal and ex vivo imaging of animals. P Damberg, C Sandberg, L Lu, J Larsson, K Edvardsson, V Siderova, Z Sarnayi, R Frias, A-C Eklöf, P Jarvoll. StratNeuro retreat. Stockholm, Sweden. 2024.
14. Frias R and Franco NH. Education and Training in Laboratory Animal Science in the European Union: Benefits, Challenges, and Solutions for Mutual Recognition. 12th World Congress on Alternatives and Animal Use in the Life Sciences. Niagara Falls, Canada. 2023. [Oral Presentation].
15. Tiebosch IA, Frias R, Franco NH. Training assessors in establishing competency of staff using animals for scientific purposes. 12th World Congress on Alternatives and Animal Use in Life Sciences. Niagara Falls, Canada. 2023. [Poster Presentation].
16. Ortin-Piqueras V, Freitag T, Spillmann T, Frias R. Development of an in vivo intestinal permeability test to refine and standardize its methodology in the laboratory mice. ESLAV-ECLAM Annual Meeting. Tallinn, Estonia. 2023. [Oral Presentation].
17. Mphande B, Costa A, Fragkiadi E, Frias R, Whitfield L. Assuring competence in practical tasks- ETPLAS working group 4. Scand-LAS international meeting. Uppsala, Sweden. 2023.
18. Alkema W, Frias R, Hijlkema N, Ritskes-Hoitinga M, Ulfhake B. 3R ranker: an artificial intelligence-based algorithm to identify replacement alternatives. FELASA 15th Congress. Marseille, France. 2022.
19. Frias R, Berg I, Ulfhake B (2019). Skills Achieved to Carry Out Procedures on Mice and Rats After Initial Practical Training. FELASA 14th Congress. Prague, Czechia. 2019.
20. Ortín Piqueras V, Freitag T, Andersson L, Lehtonen SH, Spillmann T, Meri S, Frias R (2019). Iohexol as an intestinal permeability marker in a mouse model of intestinal inflammation. FELASA 14th Congress. Prague, Czechia. 2019.
21. Forsgård RA, Marrachelli VG, Holma R, Lindén J, Frias R, Collado MC, Korpela R, Monleón D, Spillmann T, Österlund P (2017). Chemotherapy-induced gastrointestinal toxicity is associated with changes in intestinal permeability, fecal microbiota, and the metabolome in Sprague-Dawley rats. 3rd International Symposium on Profiling. Lisbon, Portugal. 2017.
22. Hillegaart V, Frias R, Wilbertz J, Ulfhake B (2017). Services for animal research around the 3Rs at Comparative Medicine, Karolinska Institutet, Sweden. 10th World Congress on the 3Rs. Seattle, USA. 2017.
23. Hillegaart V, Frias R, Wilbertz J, Ulfhake B (2017). A common set of values and the 3Rs at Karolinska Institutet, Sweden. 10th World Congress on the 3Rs. Seattle, USA. 2017.
24 Frias R (2017). Societal Attitudes to Animal Research. Human Brain Project. Research, Ethics and Societal Impact workshop. Stockholm, Sweden.
25 Frias R (2017). Management of competence for personnel working with animals at Karolinska Institutet. Spanish Society of Laboratory Animal Science 14th National Meeting. Las Palmas de Gran Canaria, Spain.
26 Ortín Piqueras V, Wilbertz J, Ulfhake B, Frias R (2017). The barrier concept of rodent facilities at Karolinska Institutet. Spanish Society of Laboratory Animal Science 14th National Meeting. Las Palmas de Gran Canaria, Spain.
27. Ortín Piqueras V, Wilbertz J, Ulfhake B, Frias R (2017). Implementation of the barrier concept of rodent facilities at Comparative Medicine, Karolinska Institutet: Wallenberg laboratory animal facility model. Spanish Society of Laboratory Animal Science 14th national meeting. Las Palmas de Gran Canaria, Spain.
28. Forsgård RA, Korpela R, Holma R, Lindén J, Frias R, Spillmann T, Österlund P (2016). Chemotherapy-induced gastrointestinal toxicity is associated with increased intestinal permeability to iohexol in Sprague-Dawley rats. EGHS-WGO International Congress, Abu Dhabi, United Arab Emirates.
29. Watson WE, Jacob ME, Bruno-Bárcena JM, Amirsultan S, Frias R, Gookin JL (2016). Abnormal Intestinal Function in Kittens Experimentally Infected With Enteropathogenic E. coli. American Society for Microbiology (ASM) Annual Meeting. Boston, MA, USA.
30. Ortín Piqueras V, Spillmann T, Pöytäkangas M, Sankari S, Frias R (2016). Validation of an enzyme-linked immunosorbent assay for measuring iohexol in canine plasma as a marker for intestinal permeability and glomerular filtration rate. ECVIM-CA 26th Annual Congress. Gothenburg, Sweden.
31. Ortín Piqueras V, Kalliokoski S, Lindfors K, Frias R (2016). Pharmacokinetics of iohexol in urine of mice following a single oral administration: identification of the optimal time-points for urine collection during the development of intestinal permeability tests in mice. FELASA 13th Congress. Brussels, Belgium.
32. Yatkin E, Frias R, Kulmala N, Major M, Jaakkola U-M (2016). ELLI Record-Keeping System as a Tool for Assessing, Reporting and Enhancing Animal Welfare at the University of Turku, Central Animal Laboratory. FELASA 13th Congress. Brussels, Belgium.
33. Ortín Piqueras V, Koskinen M, Hewetson M, Spillmann T, Pöytäkangas M, Sankari S, Vaccaro DE, Frias R (2015). Measurement of iohexol from equine plasma: comparative analysis between enzyme-linked immunosorbent assay and high performance liquid chromatography. Scand-LAS 45th Annual Congress. Turku, Finland.
34. Ortín Piqueras V, Spillmann T, Pöytäkangas M, Honkavaara JM, Sankari S, Vaccaro DE, Frias R (2015). Measurement of iohexol from canine plasma: comparative analysis between enzyme-linked immunosorbent assay, neutron activation analysis and high performance liquid chromatography. Scand-LAS 45th Annual Congress. Turku, Finland. Award prize: Top 5 Best Scientific Abstracts.
35. Kalliokoski S, Sulic A-M, Caja S, Frias R, Laurila K, Mäki M, Kaukinen K, Sblattero D, Korponay-Szabó IR, Lindfors K (2015). Injection of TG2-autoantibodies induces mild enteropathy in mice but not clinical symptoms. 16th International Coeliac Disease Symposium (Prague, Czech Republic).
36. Gómez-Gallego C, Collado MC, García-Romo M, Bernal MJ, Pérez-Martínez G , Frias R, Salminen S, Periago MJ, Ros G (2015). Integrative analysis of the effect of an infant formula enriched in polyamines on the microbiome and host changes during lactation in Balb/c mice model. 48rd Annual Meeting of the European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN).
37. Chrusciel P, Frias R, Hiltula-Maisala T, Jaakkola U-M (2014). Supervised and tailored practical instruction in handling laboratory animals and performing procedures as a way to ensure refinement. Scand-LAS 44th Annual Congress. Stockholm, Sweden.
38. Kalliokoski S, Caja S, Frias R, Laurila K, Koskinen O, Mäki M, Kaukinen K, Korponay-Szabó IR, Lindfors K (2013). Injection of celiac disease patients’ serum or immunoglobulins to mice reproduces a condition mimicking early developing celiac disease. BioMediTech, Institute of Biosciences and Medical Technology, Tampere University of Technology and University of Tampere, Finland.
39. Gómez-Gallego C, Frias R, Pérez-Martínez G, Bernal MJ, Periago MJ, Salminen S, Ros G, Collado MC (2013). Comparison in the development of the microbiota along the gastrointestinal tract of infant mice after breast- and formula feeding. IUNS 20th International Congress of Nutrition, Granada, Spain.
40. Spillmann T and Frias R (2012). Assessment of intestinal permeability in dogs. Lissabon, Portugal.
41. Gómez-Gallego C, Collado MC, Perez-Martinez G, Bernal MJ, Periago MJ, Frias R, Ros G, Salminen S (2012). Impact of infant formula supplemented with polyamines on Akkermansia muciniphila, a mucus degrading bacteria related with allergy, is influenced in BALB/cOlaHsd mice model. International Symposium on Probiotics, Prebiotics and Health, Turku, Finland.
42. Frias R, Ouwehand AC, Jaakkola U-M, Spillmann T and Gueimonde M (2011). An in vivo permeability test protocol using iohexol to reduce and refine the use of laboratory rats in intestinal damage assessment. Scand-LAS annual meeting. Copenhagen, Denmark.
43. Ilo T, Gomez-Gallego C, Jaakkola U-M, Salminen S, Ros G and Frias R (2010). A method to collect high volumes of milk from mice. FELASA/Scand-LAS Congress. Helsinki, Finland.
44. Gomez-Gallego C, Ilo T, Collado MC, Jaakkola U-M, Bernal MJ, Periago MJ, Salminen S, Ros G and Frias R (2010). Effects on the immune system of young mice fed with infant formulas supplemented with polyamines. 43rd Annual Meeting of the European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN). Istanbul, Turkey. Journal of Pediatric Gastroenterology and Nutrition, Vol: 50, pp: E196-E197, Suppl. 2, Jun 2010.
45. Frias R, Harmoinen J, Spillmann T, Sankari S and Westermarck E (2007). Permanent jejunal fistulas do not affect the small intestinal absorption or permeability in Beagle dogs. FELASA congress, Cernobbio, Italy.
46. Frias R, Sankari S, Spillmann T, Westermarck E (2005). 51Cr-EDTA permeability blood test in dogs can be equally performed in serum or plasma. ECVIM-CA/ESVIM Congress Glasgow, UK.
47. Frias R, Strube K, Bergmann K, Ternes W, Sankari S, Spillmann T, Westermarck E (2005). Comparison of 51Cr-EDTA and iohexol as intestinal permeability markers in dogs. ECVIM-CA/ESVIM Congress Glasgow, UK.
48. Klenner S, Bergmann K, Hweicker-Trautwein M, Coenen M, Ternes W, Frias R, Spillmann T (2005). Determination of iohexol canine serum for the assessment of intestinal permeability. ECVIM-CA/ESVIM Congress Glasgow, UK.
49. Frias R, Sankari S, Westermarck E (2002). 51Cr-EDTA absorption test in serum: an easier method of assessing small intestinal permeability in dogs. ECVIM-CA/ ESVIM Annual Congress. Journal of Veterinary Internal Medicine 200; 16: 632, Munich, Germany. Award prize: Top 3 Best Scientific Abstracts (Oral Presentation).
50. Harmoinen J, Heikkilä M, Frias R, Lindevall K, Westermarck E (2002). Targeted recombinant β-Lactamase (TRBL) prevents ampicillin induced changes on fecal microflora. International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM), Helsinki, Finland.
51. Harmoinen J, Heikkilä M, Frias R, Lindevall K, Westermarck E (2002). A novel treatment modality, targeted recombinant β-Lactamase (TRBL) in prevention of ampicillin induced changes on canine fecal microflora. Finnish National Veterinary Congress, Helsinki, Finland.
52. Harmoinen J, Heikkilä M, Frias R, Lindevall K, Koski P, Heino P, Westermarck E (2002). Targeted recombinant β-Lactamase (TRBL) prevents ampicillin induced changes on fecal microflora; studied by 16S rRNA fingerprints. Annual Meeting of the Scandinavian Society for Antimicrobial Chemotherapy, Turku, Finland.
Book chapters, working reports, and other publications
1. Frias R and Flecknell P (2024). Education, Training, and Competence in Laboratory Animal Science. In: Guillén, J., Galligioni, V. (eds) Practical Management of Research Animal Care and Use Programs. Laboratory Animal Science and Medicine, vol 3. Springer, Cham. https://doi.org/10.1007/978-3-031-65414-5_13
2. Pastor Campos A, de la Cueva Bueno E, Martín Zúñiga J, Frias R and Muñoz Mediavilla C. (2018). Guidelines for Persons Working Under Supervision in Laboratory Animal Facilities. Journal of the Spanish Society of Laboratory Animal Science Association (JSECAL). [Working Group Report]
3. Frias R (2009). Laboratory animal models in research. In Y. K. Lee & S. Salminen (Eds.), Handbook of probiotics and prebiotics (2nd ed., pp. 263–267). Hoboken, NJ: John Wiley & Sons. https://doi.org/10.1002/9780470432624 [Book Chapter]
4. Lahtinen, S. J., Boyle, R. J., Margolles, A., Frias, R., & Gueimonde, M. (2009). Safety assessment of probiotics. In D. Charalampopoulos & R. A. Rastall (Eds.), Prebiotics and probiotics science and technology (pp. 1193–1235). Springer. https://doi.org/10.1007/978-0-387-79058-9_31 [Book Chapter]
5. Frias R (2007). Overview of the new legislation on animal experiments in Finland. ESLAV Briefing (2007) May; 10 (I): 3-4.
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